Journal: Molecular Oncology

Article Title: Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

doi: 10.1002/1878-0261.13763

Figure Lengend Snippet: Subcutaneous tumor changed gut microbiota diversity. (A) Flowchart of subcutaneous inoculation of a tumor. (B) The weight (left) and volume (right) of the MC38 and LLC tumor. (C) Shannon, Chao1, and ACE indices among different groups. (D) PCoA of gut microbiota collected before (spre‐MC38 group) and after (spost‐MC38 group) MC38 tumor subcutaneous inoculation, as well as before (spre‐LLC group) and after (spost‐LLC group) LLC tumor subcutaneous inoculation, at the jaccard distance. (E) PCoA of spre (combination of spre‐MC38 and spre‐LLC groups) and spost (combination of spost‐MC38 and spost‐LLC groups) groups at the jaccard distance. N = 10 per group. Statistical significance was assessed by Student's t test (C, E) or Kruskal–Wallis test (D). Data are shown as the mean ± SEM. NS, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Murine lung cancer cell line LL/2 (LLC1) (RRID: CVCL_4358) and murine colon cancer cell line MC‐38 (RRID: CVCL_B288) were obtained from iCell (Shanghai, China).

Techniques:

Journal: Molecular Oncology

Article Title: Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

doi: 10.1002/1878-0261.13763

Figure Lengend Snippet: Subcutaneous tumor changed gut microbiota composition. LDA effect size (LEfSe) showed a significant difference in abundance (LDA score > 2) at phylum (A) and species level (B). (C) Distribution of ligilactobacillus and Lactobacillus among feces collected before (spre‐MC38 group) and after (spost‐MC38 group) MC38 tumor subcutaneous inoculation, as well as before (spre‐LLC group) and after (spost‐LLC group) LLC tumor subcutaneous inoculation. (D) Log ratio of Firmicutes / Bacteroidetes (F/B) among four groups. N = 10 per group. Statistical significance was assessed by Tukey's multiple comparisons in one‐way ANOVA. Data are shown as the mean ± SEM. NS, not significant. ** P < 0.01.

Article Snippet: Murine lung cancer cell line LL/2 (LLC1) (RRID: CVCL_4358) and murine colon cancer cell line MC‐38 (RRID: CVCL_B288) were obtained from iCell (Shanghai, China).

Techniques:

Journal: Molecular Oncology

Article Title: Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

doi: 10.1002/1878-0261.13763

Figure Lengend Snippet: Metastatic tumors significantly altered the gut microbiota. (A) Flowchart of the construction of metastatic tumor model. (B)Shannon, Chao1, and ACE indices among different groups. (C) PCoA of gut microbiota collected before (mpre‐MC38 group) and after (mpost‐MC38 group) MC38 tumor intravenous inoculation, as well as before (mpre‐LLC group) and after (mpost‐LLC group) LLC tumor intravenous inoculation, at the jaccard distance. (D) PCoA of mpre (combination of mpre‐MC38 and mpre‐LLC groups) and mpost (combination of mpost‐MC38 and mpost‐LLC groups) groups at the jaccard distance. (E) Distribution of Bacteroidota , Bacillota , and Pseudomonadota (left) among four groups as well as distribution of Ligilactobacillus , Lactobacillus , and Duncaniella (right) among four groups. (F) Log ratio of Firmicutes / Bacteroidetes (F/B) among four groups. N = 7 for MC38 group and N = 8 for LLC group. Statistical significance was assessed by Student's t test (B, D) or Tukey's multiple comparisons in one‐way ANOVA (E, F) or Kruskal–Wallis test (C). Data are shown as the mean ± SEM. NS, not significant. * P < 0.05, ** P < 0.01, *** P < 0.001, **** P < 0.0001.

Article Snippet: Murine lung cancer cell line LL/2 (LLC1) (RRID: CVCL_4358) and murine colon cancer cell line MC‐38 (RRID: CVCL_B288) were obtained from iCell (Shanghai, China).

Techniques:

Journal: Molecular Oncology

Article Title: Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

doi: 10.1002/1878-0261.13763

Figure Lengend Snippet: Enriched KEGG pathway modules of metabolic function of gut microbiota in post tumor inoculation groups. (A) Enriched modules in gut microbiota collected after MC38 (spost‐MC38 group) and LLC (spost‐LLC group) tumor subcutaneous inoculation compared with their respective pre‐inoculation groups. (B) Enriched modules in gut microbiota collected after MC38 (mpost‐MC38 group) and LLC (mpost‐LLC group) tumor intravenous inoculation compared with their respective pre‐inoculation groups. (C) Veen diagram of enriched modules among four groups.

Article Snippet: Murine lung cancer cell line LL/2 (LLC1) (RRID: CVCL_4358) and murine colon cancer cell line MC‐38 (RRID: CVCL_B288) were obtained from iCell (Shanghai, China).

Techniques:

Journal: Molecular Oncology

Article Title: Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

doi: 10.1002/1878-0261.13763

Figure Lengend Snippet: The similarity between subcutaneous and metastatic tumors. (A) The Shannon, Chao1, and ACE indices among different groups. (B) PCoA of gut microbiota collected after MC38 (spost‐MC38 group, n = 10) and LLC (spost‐LLC group, n = 10) subcutaneous inoculation, as well as after MC38 (mpost‐MC38 group, n = 7) and LLC (mpost‐LLC group, n = 8) intravenous inoculation, at jaccard distance (left). PCoA of spost (combination of spost‐MC38 and spost‐LLC groups) and mpost (combination of mpost‐MC38 and mpost‐LLC groups) groups at jaccard distance (right). (C) Composition of individuals collected after tumor inoculated at phylum (upper) and genus level (lower). Statistical significance was assessed by Student's t test. Data are shown as the mean ± SEM. NS, not significant.

Article Snippet: Murine lung cancer cell line LL/2 (LLC1) (RRID: CVCL_4358) and murine colon cancer cell line MC‐38 (RRID: CVCL_B288) were obtained from iCell (Shanghai, China).

Techniques:

Journal: Molecular Oncology

Article Title: Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

doi: 10.1002/1878-0261.13763

Figure Lengend Snippet: No marked colorectum mucosa injury was observed in tumor‐bearing mice. (A) Representative HE staining images of colorectum collected from control group ( n = 4), MC38 (spost‐MC38 group, n = 10), and LLC (spost‐LLC group, n = 10) subcutaneous inoculation groups, as well as from MC38 (mpost‐MC38 group, n = 7) and LLC (mpost‐LLC group, n = 8) intravenous inoculation groups. Histological score (B) and villus/crypt (C) among different groups. (D) ZO‐1 expression levels in the colorectum of different groups were visualized by immunohistochemistry. Scale bar = 250 μm. Statistical significance was assessed by Dunn's multiple comparisons in Kruskal–Wallis test (B) or Tukey's multiple comparisons in one‐way ANOVA (C). Data are shown as the mean ± SEM. NS, not significant

Article Snippet: Murine lung cancer cell line LL/2 (LLC1) (RRID: CVCL_4358) and murine colon cancer cell line MC‐38 (RRID: CVCL_B288) were obtained from iCell (Shanghai, China).

Techniques: Staining, Control, Expressing, Immunohistochemistry

Journal: Molecular Oncology

Article Title: Crosstalk between gut microbiota and tumor: tumors could cause gut dysbiosis and metabolic imbalance

doi: 10.1002/1878-0261.13763

Figure Lengend Snippet: FMT of feces from preinoculation mice reduced tumor growth. (A) Flowchart of the transplantation of feces from mice before (FMT‐spre‐MC38) and after (FMT‐spost‐MC38) inoculation of MC38 tumor, as well as before (FMT‐spre‐LLC) and after (FMT‐spost‐LLC) inoculation of LLC tumor to recipient mice, respectively. (B) Tumor volume of MC38 (left) and LLC (right) was measured every 2 days. (C) Immunohistochemistry of tumor tissue for Ki‐67 expression (scale bar = 250 μm) among different groups. One mouse in the FMT‐spost‐MC38 group and one in the FMT‐spost‐LLC group died during gavage process. Statistical significance was assessed by Tukey's multiple comparisons in one‐way ANOVA. Data are shown as the mean ± SEM. N = 5 per group. NS, not significant. * P < 0.05

Article Snippet: Murine lung cancer cell line LL/2 (LLC1) (RRID: CVCL_4358) and murine colon cancer cell line MC‐38 (RRID: CVCL_B288) were obtained from iCell (Shanghai, China).

Techniques: Transplantation Assay, Immunohistochemistry, Expressing